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KMID : 0352719930170020139
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Journal of Ginseng Research
1993 Volume.17 No. 2 p.139 ~ p.144
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Colorimetric Determination of Acidic Polysaccharide from panax ginseng, its Extraction Condition and Stability
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도재호
이형옥/이성계/장진규/이성동/성현순
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Abstract
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The method for colorimetric determination of acidic polysaccharide from Panax ginseng was investigated. It is possible to apply the method of carbazole- sulfuric acid to determination of pectin, and also to measure the amount of pectin in the
mixture of
various high molecular compounds such as starch, cellulose and gum, etc. When the method of carbazole-sulfuric acid was applied to determine the amount of acidic polysaccharide, optical density at 525 nm increased linearly with an increase in the
concentration of pure acidic polysaccharide. Effective extraction temperature with water for the determination of the amount of ginseng acidic polysaccharide (GAP) was 80℃. In order to separate or concentrate GAP, it was appropriate to
precipitate
the
extract only once with 80% ethyl alcohol GAP was very stable at 100℃ for 4 hrs in aqueous solution and between pH values of 5.0~12.0
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KEYWORD
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